Electrokinetic Library of Microorganisms

Microscale BioSeparations Laboratory

 

EEEC= Electrokinetic Equilibrium Condition, which is to be used as an EK signature for each type of microorganism for the design of insulator-based separation systems. For more information on how to use this parameter please refer to this article [1].

Publication on bacterial and yeast cells [2].

Publication on bacteriophage virus [3].

 

Microorganism

Size

EEEC (V/cm)

Media conditions

Bacterial cells

 

Bacillus cereus

ATCC #14579

Length: 4.94±0.47 (mm)

Width: 1.32±0.13 (mm)

618 ± 32

DI water

Cond =13.44 ± 0.07 mS/cm

pH = 6.78 ± 0.61

Bacillus subtilis

ATCC #6051

Length: 4.86±0.41 (mm)

Width: 1.94±0.19 (mm)

545 ± 23

DI water

Cond =13.44 ± 0.07 mS/cm

pH = 6.78 ± 0.61

Escherichia coli

ATCC #11775

Length: 2.38±0.32 (mm)

Width: 0.96±0.21 (mm)

1,092 ± 76

DI water

Cond =13.44 ± 0.07 mS/cm

pH = 6.78 ± 0.61

Escherichia coli

ATCC #25922

Length: 2.01±0.42 (mm)

Width: 0.97±0.21 (mm)

1,071 ± 13

DI water

Cond =12.43 ± 1.36 mS/cm

pH = 6.86 ± 0.71

Salmonella enterica serovar Typhimurium (TT9079)

Length: 2.00±0.31 (mm)

Width: 0.97±0.11 (mm)

1,928 ± 170

DI water

Cond =12.35 ± 1.58 mS/cm

pH = 6.88 ± 0.74

Yeast cells

Saccharomyces cerevisiae

ATCC #9763

Diameter: 6.23±0.77 (mm)

505 ± 21

DI water

Cond =13.44 ± 0.07 mS/cm

pH = 6.78 ± 0.61

Bacteriophages

P. chlororaphis phage 201φ2-1

200 nm diam. head, 20x211 nm tail

3,095 ± 238

DI water

Cond =23.73 ± 12.02 mS/cm

pH = 7.03 ± 0.58

 Salmonella Typhimurium Phage φKZ

 

145 nm diam. head, 22x200 nm tail

2,140 ± 107

DI water

Cond =15.89 ± 6.65 mS/cm

pH = 6.57 ± 0.35

 Pseudomonas aeruginosa phage SPN3US

60 nm diam. head, 18x35 nm tail

2,932 ± 247

DI water

Cond =11.97 ± 0.31 mS/cm

pH = 6.41 ± 0.13

References:

1.            Coll De Peña, A., N. Hill, and B.H. Lapizco-Encinas, Fine-tuning the determination of the electrokinetic equilibrium condition of microorganisms. In preparation, to be submitted, 2020.

2.            Coll De Peña, A., A. Miller, C.J. Lentz, N. Hill, A. Parthasarathy, A.O. Hudson, and B.H. Lapizco-Encinas, Creation of an electrokinetic characterization library for the detection and identification of biological cells. Analytical and Bioanalytical Chemistry, 2020. DOI: 10.1007/s00216-020-02621-9.

3.            Coll De Peña, A., N.H. Mohd Redzuan, M.K. Abajorga, N. Hill, J.A. Thomas, and B.H. Lapizco-Encinas, Analysis of Bacteriophages with Insulator-Based Dielectrophoresis. Micromachines, 2019. 10(7): p. 450.