Electrokinetic Library of Microorganisms

Microscale BioSeparations Laboratory

eE_EEC= empirical Electrokinetic Equilibrium Condition, which is to be used as an EK signature for each type of microorganism for the design of insulator-based separation systems.

· For more information on how to use the eE_EEC please refer to this article [1].

· Publication on the derivation of the parameter E_EEC [2]

· Publication on bacterial and yeast cells [3].

· Publication on bacteriophage virus [4].

Microorganism	Size	eEEEC (V/cm)	Media conditions
Bacterial cells
Bacillus cereus ATCC #14579	Length: 4.94±0.47 (mm) Width: 1.32±0.13 (mm)	618 ± 32	DI water Cond =13.44 ± 0.07 mS/cm pH = 6.78 ± 0.61
Bacillus subtilis ATCC #6051	Length: 4.86±0.41 (mm) Width: 1.94±0.19 (mm)	545 ± 23	DI water Cond =13.44 ± 0.07 mS/cm pH = 6.78 ± 0.61
Escherichia coli ATCC #11775	Length: 2.38±0.32 (mm) Width: 0.96±0.21 (mm)	1,092 ± 76	DI water Cond =13.44 ± 0.07 mS/cm pH = 6.78 ± 0.61
Escherichia coli ATCC #25922	Length: 2.01±0.42 (mm) Width: 0.97±0.21 (mm)	1,071 ± 13	DI water Cond =12.43 ± 1.36 mS/cm pH = 6.86 ± 0.71
Salmonella enterica serovar Typhimurium (TT9079)	Length: 2.00±0.31 (mm) Width: 0.97±0.11 (mm)	1,928 ± 170	DI water Cond =12.35 ± 1.58 mS/cm pH = 6.88 ± 0.74
Yeast cells
Saccharomyces cerevisiae ATCC #9763	Diameter: 6.23±0.77 (mm)	505 ± 21	DI water Cond =13.44 ± 0.07 mS/cm pH = 6.78 ± 0.61
Bacteriophages
P. chlororaphis phage 201φ2-1	129 nm diam. head, 200x20 nm tail Source: [5]	3,095 ± 238	DI water Cond =23.73 ± 12.02 mS/cm pH = 7.03 ± 0.58
Pseudomonas aeruginosa Phage φKZ	145 nm diam. head, 200x20 nm tail Source: [6]	2,140 ± 107	DI water Cond =15.89 ± 6.65 mS/cm pH = 6.57 ± 0.35
Salmonella enterica Typhimurium phage SPN3US	140 nm diam. head, 180x20 nm tail Source: [7, 8]	2,932 ± 247	DI water Cond =11.97 ± 0.31 mS/cm pH = 6.41 ± 0.13

References:

1. Coll De Peña A, Hill N, Lapizco-Encinas BH (2020) Determination of the Empirical Electrokinetic Equilibrium Condition of Microorganisms in Microfluidic Devices. Biosensors 10:148 . doi: 10.3390/bios10100148

2. Cardenas-Benitez B, Jind B, Gallo-Villanueva RC, Martinez-Chapa SO, Lapizco-Encinas BH, Perez-Gonzalez VH (2020) Direct Current Electrokinetic Particle Trapping in Insulator-Based Microfluidics: Theory and Experiments. Anal Chem 92:12871–12879 . doi: 10.1021/acs.analchem.0c01303

3. Coll De Peña A, Miller A, Lentz CJ, Hill N, Parthasarathy A, Hudson AO, Lapizco-Encinas BH (2020) Creation of an electrokinetic characterization library for the detection and identification of biological cells. Anal Bioanal Chem 412:3935–3945 . doi: 10.1007/s00216-020-02621-9

4. Coll De Peña A, Mohd Redzuan NH, Abajorga M, Hill N, Thomas JA, Lapizco-Encinas BH (2019) Analysis of bacteriophages with insulator-based dielectrophoresis. Micromachines 10:450 . doi: 10.3390/mi10070450

5. Yuan Y, Gao M (2017) Jumbo Bacteriophages: An Overview. Jumbo Bacteriophages An Overview Front Microbiol 8:403 . doi: 10.3389/fmicb.2017.00403

6. Sokolova OS, Shaburova O V., Pechnikova E V., Shaytan AK, Krylov S V., Kiselev NA, Krylov VN (2014) Genome packaging in EL and Lin68, two giant phiKZ-like bacteriophages of P. aeruginosa. Virology 468:472–478 . doi: 10.1016/j.virol.2014.09.002

7. Ali B, Desmond MI, Mallory SA, Benítez AD, Buckley LJ, Weintraub ST, Osier M V., Black LW, Thomas JA (2017) To be or not to be T4: Evidence of a complex evolutionary pathway of head structure and assembly in giant Salmonella virus SPN3US. Front Microbiol 8:2251 . doi: 10.3389/fmicb.2017.02251

8. Heymann JB, Wang B, Newcomb WW, Wu W, Winkler DC, Cheng N, Reilly ER, Hsia R-C, Thomas JA, Steven AC (2020) The Mottled Capsid of the Salmonella Giant Phage SPN3US, a Likely Maturation Intermediate with a Novel Internal Shell. Viruses 12:910 . doi: 10.3390/v12090910